At least one dilution will result in well separated colonies, enabling us to collect isolates.Īpplications. Rather than try to guess how much we need to dilute the sample, we spread agar plates with progressively more dilute samples. The concentration of bacteria in a typical undiluted sample is so high that colonies growing on an agar plate prepared from that sample will be far too crowded to permit identification and isolation of unique colony types. We then isolate cultures from the colonies that grow on the agar surface. A conventional way to obtain bacterial isolates from an environmental sample is to prepare a liquid suspension of the sample, make serial dilutions, then spread a small volume of each dilution onto one or more agar plates.
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